Determination for Artemia Cysts Quality 2009-4-1
1.Hatch Rate (HR%)
This is ascertained by first finding out the Cyst Per Gram (CPG) count and then finding out the Nauplii Per Gram count and dividing the two as NPG / CPG.
This number is crucial because it is the industry standard by which brine shrimp eggs are sold. However, as we are dealing with a living organism, there are inherent differences with the physical properties of brine shrimp eggs. Variations that may affect the HR % can be due to many factors including the source location of the eggs, the biological strain of the eggs, processing methods, and so on.
2.Nauplii Per Gram (NPG)
This is calculated by obtaining the total number of newly hatched nauplii within the hatching container and dividing this number by the number of grams of brine shrimp eggs that were put into the hatching container at first.
This number is important because this is the number of brine shrimp nauplii individuals that the customer is getting. It is also significant because this is the end product, i.e., this is what will be consumed by the fish or shrimp larvae. Many hatchery technicians base their feeding regimes on the NPG.
3. Separation
This is a subjective indicator determined by the person in charge of hatching out the brine shrimp eggs. It is derived by first visually observing a sample of approximately 500-1000 ml in an imhoff cone or glass beaker from the hatching container immediately prior to harvesting the brine shrimp nauplii to note the separation of newly hatched brine shrimp nauplii from un-hatched eggs and shells. With excellent separation, one should observe mostly newly hatched brine shrimp nauplii, with little to none eggs or shells on the bottom of the cone or beaker.
This step is important because the introduction of un-hatched eggs and shells into the larval rearing tanks can cause water to be impure, leading to health related problems with the cultured organisms.